Crystal Structure of the Marburg Virus GP2 Core Domain in its Post-Fusion Conformation†
Identifieur interne : 000B80 ( Main/Exploration ); précédent : 000B79; suivant : 000B81Crystal Structure of the Marburg Virus GP2 Core Domain in its Post-Fusion Conformation†
Auteurs : Jayne F. Koellhoffer [États-Unis] ; Vladimir N. Malashkevich [États-Unis] ; Joseph S. Harrison [États-Unis] ; Rafael Toro [États-Unis] ; Rahul C. Bhosle [États-Unis] ; Kartik Chandran [États-Unis] ; Steven C. Almo [États-Unis] ; Jonathan R. LaiSource :
- Biochemistry [ 0006-2960 ] ; 2012.
Abstract
Marburg virus (MARV) and Ebola virus (EBOV) are members of the family
Url:
DOI: 10.1021/bi300976m
PubMed: 22935026
PubMed Central: 3464016
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000490
- to stream Pmc, to step Curation: 000490
- to stream Pmc, to step Checkpoint: 000518
- to stream Ncbi, to step Merge: 000291
- to stream Ncbi, to step Curation: 000291
- to stream Ncbi, to step Checkpoint: 000291
- to stream Main, to step Merge: 000B82
- to stream Main, to step Curation: 000B80
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Crystal Structure of the Marburg Virus GP2 Core Domain in its Post-Fusion Conformation<xref ref-type="fn" rid="FN1">†</xref>
</title>
<author><name sortKey="Koellhoffer, Jayne F" sort="Koellhoffer, Jayne F" uniqKey="Koellhoffer J" first="Jayne F." last="Koellhoffer">Jayne F. Koellhoffer</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Malashkevich, Vladimir N" sort="Malashkevich, Vladimir N" uniqKey="Malashkevich V" first="Vladimir N." last="Malashkevich">Vladimir N. Malashkevich</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Harrison, Joseph S" sort="Harrison, Joseph S" uniqKey="Harrison J" first="Joseph S." last="Harrison">Joseph S. Harrison</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Toro, Rafael" sort="Toro, Rafael" uniqKey="Toro R" first="Rafael" last="Toro">Rafael Toro</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Bhosle, Rahul C" sort="Bhosle, Rahul C" uniqKey="Bhosle R" first="Rahul C." last="Bhosle">Rahul C. Bhosle</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Chandran, Kartik" sort="Chandran, Kartik" uniqKey="Chandran K" first="Kartik" last="Chandran">Kartik Chandran</name>
<affiliation wicri:level="2"><nlm:aff id="A2">Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Almo, Steven C" sort="Almo, Steven C" uniqKey="Almo S" first="Steven C." last="Almo">Steven C. Almo</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Lai, Jonathan R" sort="Lai, Jonathan R" uniqKey="Lai J" first="Jonathan R." last="Lai">Jonathan R. Lai</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">22935026</idno>
<idno type="pmc">3464016</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464016</idno>
<idno type="RBID">PMC:3464016</idno>
<idno type="doi">10.1021/bi300976m</idno>
<date when="2012">2012</date>
<idno type="wicri:Area/Pmc/Corpus">000490</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000490</idno>
<idno type="wicri:Area/Pmc/Curation">000490</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000490</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000518</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000518</idno>
<idno type="wicri:Area/Ncbi/Merge">000291</idno>
<idno type="wicri:Area/Ncbi/Curation">000291</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000291</idno>
<idno type="wicri:doubleKey">0006-2960:2012:Koellhoffer J:crystal:structure:of</idno>
<idno type="wicri:Area/Main/Merge">000B82</idno>
<idno type="wicri:Area/Main/Curation">000B80</idno>
<idno type="wicri:Area/Main/Exploration">000B80</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Crystal Structure of the Marburg Virus GP2 Core Domain in its Post-Fusion Conformation<xref ref-type="fn" rid="FN1">†</xref>
</title>
<author><name sortKey="Koellhoffer, Jayne F" sort="Koellhoffer, Jayne F" uniqKey="Koellhoffer J" first="Jayne F." last="Koellhoffer">Jayne F. Koellhoffer</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Malashkevich, Vladimir N" sort="Malashkevich, Vladimir N" uniqKey="Malashkevich V" first="Vladimir N." last="Malashkevich">Vladimir N. Malashkevich</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Harrison, Joseph S" sort="Harrison, Joseph S" uniqKey="Harrison J" first="Joseph S." last="Harrison">Joseph S. Harrison</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Toro, Rafael" sort="Toro, Rafael" uniqKey="Toro R" first="Rafael" last="Toro">Rafael Toro</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Bhosle, Rahul C" sort="Bhosle, Rahul C" uniqKey="Bhosle R" first="Rahul C." last="Bhosle">Rahul C. Bhosle</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Chandran, Kartik" sort="Chandran, Kartik" uniqKey="Chandran K" first="Kartik" last="Chandran">Kartik Chandran</name>
<affiliation wicri:level="2"><nlm:aff id="A2">Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Almo, Steven C" sort="Almo, Steven C" uniqKey="Almo S" first="Steven C." last="Almo">Steven C. Almo</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Lai, Jonathan R" sort="Lai, Jonathan R" uniqKey="Lai J" first="Jonathan R." last="Lai">Jonathan R. Lai</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Biochemistry</title>
<idno type="ISSN">0006-2960</idno>
<idno type="eISSN">1520-4995</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">Marburg virus (MARV) and Ebola virus (EBOV) are members of the family <italic>Filoviridae</italic>
(‘filoviruses’) that cause severe hemorrhagic fever with human case fatality rates of up to 90%. Filovirus infection requires fusion of the host cell and virus membranes, a process that is mediated by the envelope glycoprotein (GP). GP contains two subunits, the surface subunit (GP1), which is responsible for cell attachment, and the transmembrane subunit (GP2), which catalyzes membrane fusion. The GP2 ectodomain contains two heptad repeat regions, N-terminal and C-terminal (NHR and CHR, respectively) that adopt a six-helix bundle during the fusion process. The refolding of this six-helix bundle provides the thermodynamic driving force to overcome barriers associated with membrane fusion. Here we report the crystal structure of the MARV GP2 core domain in its post-fusion (six-helix bundle) conformation at 1.9 Å resolution. The MARV GP2 core domain backbone conformation is virtually identical to that of EBOV GP2 (reported previously), and consists of a central NHR core trimeric coiled-coil packed against peripheral CHR α-helices and an intervening loop/helix-turn-helix segment. We previously reported that the stability of the MARV GP2 post-fusion structure is highly pH-dependent, with increasing stability at lower pH [Harrison, J.S.; Koellhoffer, J. K.; Chandran, K.; and Lai, J. R. <italic>Biochemistry</italic>
, 2012, <italic>51</italic>
, 2515–2525]. We hypothesized that this pH-dependent stability provides a mechanism for conformational control such that the post-fusion six helix bundle is promoted in the environments of appropriately matured endosomes. In this report, a structural rationale for this pH-dependent stability is described, and involves a high-density array of core and surface acidic side chains at the midsection of the structure, termed the ‘anion stripe.’ In addition, many surface-exposed salt bridges likely contribute to stabilizing the post-fusion structure at low pH. These results provide structural insights into the mechanism of MARV GP2-mediated membrane fusion.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>État de New York</li>
</region>
</list>
<tree><noCountry><name sortKey="Lai, Jonathan R" sort="Lai, Jonathan R" uniqKey="Lai J" first="Jonathan R." last="Lai">Jonathan R. Lai</name>
</noCountry>
<country name="États-Unis"><region name="État de New York"><name sortKey="Koellhoffer, Jayne F" sort="Koellhoffer, Jayne F" uniqKey="Koellhoffer J" first="Jayne F." last="Koellhoffer">Jayne F. Koellhoffer</name>
</region>
<name sortKey="Almo, Steven C" sort="Almo, Steven C" uniqKey="Almo S" first="Steven C." last="Almo">Steven C. Almo</name>
<name sortKey="Bhosle, Rahul C" sort="Bhosle, Rahul C" uniqKey="Bhosle R" first="Rahul C." last="Bhosle">Rahul C. Bhosle</name>
<name sortKey="Chandran, Kartik" sort="Chandran, Kartik" uniqKey="Chandran K" first="Kartik" last="Chandran">Kartik Chandran</name>
<name sortKey="Harrison, Joseph S" sort="Harrison, Joseph S" uniqKey="Harrison J" first="Joseph S." last="Harrison">Joseph S. Harrison</name>
<name sortKey="Malashkevich, Vladimir N" sort="Malashkevich, Vladimir N" uniqKey="Malashkevich V" first="Vladimir N." last="Malashkevich">Vladimir N. Malashkevich</name>
<name sortKey="Toro, Rafael" sort="Toro, Rafael" uniqKey="Toro R" first="Rafael" last="Toro">Rafael Toro</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B80 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000B80 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV1 |flux= Main |étape= Exploration |type= RBID |clé= PMC:3464016 |texte= Crystal Structure of the Marburg Virus GP2 Core Domain in its Post-Fusion Conformation† }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22935026" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a CovidV1
This area was generated with Dilib version V0.6.33. |